Lab Protocols
Bacteria
- DH5 Alpha Competent Cells
- E-media recipe for 50X E stock
- Purification of bacterial chromosomal DNA for PCR
- Bacterial Transformation - Electroporation
- Electroporation - competent cells, Electroporation
- E. coli Transformation
- Linear Transformation Salmonella
- NaNO3 mutagenesis
- Phage P22
- Plasmid Prep
- Transposon Pools
Basic Lab Protocols
- 96 Well Template
- Common Stock Solution Recipes
- General Lab Etiquette
- Glass Pipette Cleaning
- Pipetting Exercise
- Protocol Notebook Entry
PCR-Cloning
- Blunt Cloning
- Cloning Protocol
- Colony PCR from Yeast or Bacteria
- MCS Cloning
- Multi-site Directed Mutagenesis
- PCR Machines
- PCR Protocol
- Random Mutagenesis PCR
- Sequencing Reaction Protocol
- Single Primer Protocol
Proteins
- 6x Sample Buffer
- Bacterial His tag purification
- Buffers for Western Blot
- Coomassie Stain Protocol
- Dirty TAP PSK Purification from Yeast
- GST Protein Purification
- HIs6-yUGP1 purification from Bacteria
- hPASKFL Purification
- IP of hPASK from 293
- IP Kinase Assay Protocol
- TCA Protein Precipitation Protocol
- UGPase Activity Assay
- Western Blotting Protocol
- Western of hPASK
- Yeast HIS Tag Purification