SDS-Page Solutions
Lower Gel Buffer (1.5 M Tris, 0.4% SDS, pH 8.8)
- 90.9 g. Tris Base
- 2 g. SDS
- Adjust pH to 8.8
- q.s. to 500 mL w/ ddH2O
Upper Gel Buffer (0.5 M Tris, 0.4% SDS, pH 6.8)
- 30.3 g. Tris Base
- 2 g. SDS
- Adjust pH to 6.8
- q.s. to 500 mL w/ ddH2O
30% Acrylamide
- 150 g. 29.2% Acrylamide
- 4.1 g. 0.8% Bis-Acrylamide
- q.s. to 513.5 mL w/ ddH2O
- Filter sterilize; store at 4oC; store in opaque/dark bottle.