LB Derivations
LB Broth
For 2 Liters:
- 20 g. tryptone
- 10 g. yeast extract
- 20 g. NaCl
- q.s. to 2 L w/ ddH2O
- Adjust pH to 7.4 w/ NaOH
- Autoclave 45 min.
- Divide into 250 mL aliquots
LB Plates
- 500 mL LB broth (pH 7.4)
- 7.5 g. Noble Agar (15 g/L)
- Divide into 2- 250 mL bottles
- Autoclave 45 min.
- Cool to 55oC in H2O bath
- Add 500 uL ampicillin
- Pour plates, store stacked. Good for 1 month.