Agarose Gels & Buffers

2% Agarose Gel

• 100ml 1x TAE Buffer
• 2.0g Agarose
• Microwave 3 min. until clear. Can store extra in bottle.

Gel Buffer Running Solution

• 750 mL ddH₂O
• 15 mL 50x TAE
• 20 uL Ethidium Bromide

Small Gel

• 250 mL ddH₂O
• 5 mL 50x TAE
• 6.5 uL Ethidium Bromide
• 10x Ligation Buffer

[Final]

• 0.5 M Tris, pH 7.5
• 100 mM MgCl₂
• 100 mM DTT
• 0.5 mg/mL BSA
• 10 mM ATP
• 5 mM Spermidine

BSA 10x Buffer for DRA III

• 10 uL BSA
• 90 uL ddH₂O
• Vortex; place 1 uL per sample.