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College of Life Sciences Microbiology and Molecular Biology
Griffitts Lab

Protocols

DNA

Phylogenetics
PCR primers
PCR with Taq Polymerase
Overlap Extension
Arbitrary PCR
PCR clean-up (Qiagen)
Sequencing prep
Nucleobond Midiprep
Genomic DNA prep (Qiagen)
Gel electrophoresis
Modified Eckhardt gel
Restriction Digest
In-gel ligation

RNA

Trizol RNA purification (bacterial)
DNAse treatment
Reverse transcription
5' RACE

Plants

Media
Conetainer Rack Protocol
Seed scarification and germination
Tips for growth/seed storage
DNA prep
RNA prep
Seed Storage
Microscopy
Nodule Bacteria Recovery
Nodule Fixation/Embedding
LacZ Staining of Nodules

Protein

Gel recipes
Sample buffer
Bacterial lysis
6His purification
MBP purification

Recipes

Common Buffers
Common Media
Stock Solution

Bacteria

Media
Antibiotics
Tips on streaking/culturing
Freezing procedure
Genomic DNA prep (Qiagen)
RNA prep (Trizol)
ß-Gal/GUS assay
LIVE/DEAD BacLight Staining
Triparental Mating
Transduction
Chemocompetent Cells
Transformation
Electrocompetent Cells
Transformation
LPS Analysis
Competition Experiments
Modified Eckhardt gel