Arbitrary PCR
Introduction
You need nested outward-pointing transposon-specific primers (TSP) within 150 bp of the transposon end. These should both be standard primers with Tm of 60–65°C. The primer more distal from the transposon end (TSP1) is to be used in the first-round PCR
Round One
Round One recipe

*For mini-Tn5:110 (NmR) use oJG133.
For mini-Tn5:267 or mini-Tn5:285 (both TcR) use oJG697.
For mini-Tn5:520 (GmR) use oJG1254.
**For amplifying rhizobium sequences, boil a dense suspension of cells in 50 μL PCR lysis buffer
Round One cycling

Note: For more info, see the standard PCR procedure
Round Two
Round Two recipe

*For mini-Tn5:110 (NmR) use oJG134.
For mini-Tn5:267 or mini-Tn5:285 (both TcR) use oJG698.
For mini-Tn5:520 (GmR) use oJG1255.
Round Two cycling

Note: For more info, see the standard PCR procedure
Conclusion
Second-round reactions are then cleaned up by Qiagen
Primers
ARB1A
GCCACGCGTCGACTAGTACNNNNNNNNNNACGCC
ARB1B
GCCACGCGTCGACTAGTACNNNNNNNNNNTGCGG
ARB1C
GCCACGCGTCGACTAGTACNNNNNNNNNNTCCGG
ARB2
GCCACGCGTCGACTAGTAC