Lab Protocol

Recipes

Common buffers
Common media
Stock solutions

DNA

Phylogenetics - PCR
PCR primers
Standard PCR
Overlap-extension PCR
Arbitrary PCR
PCR clean-up (Qiagen)
Sequencing prep - DNA Prep
Qiagen miniprep
Low-copy plasmid prep
Nucleobond Midiprep
Genomic DNA prep (Qiagen)
Gel electrophoresis
Modified Eckhardt gel
Restriction digest
In-gel ligation

RNA

Trizol RNA purification (bacterial)
DNAse treatment
Reverse transcription
5' RACE

Protein

Gel recipes
Sample buffer
Bacterial lysis
6His purification
MBP purification

Bacteria

Media
Antibiotics
Tips on streaking/culturing
Freezing procedure
Genomic DNA prep (Qiagen)
RNA prep (Trizol)
ß-Gal/GUS assay
LIVE/DEAD BacLight Staining
Triparental Mating
Transduction
Chemocompetent Cells
- Transformation
Electrocompetent Cells
- Transformation
LPS Analysis
Competition Experiments
Modified Eckhardt gel

Plants

Media
Conetainer Rack Protocol
Seed scarification and germination
Tips for growth/seed storage
DNA prep
RNA prep
Seed Storage
Microscopy
Nodule Bacteria Recovery
Nodule Fixation/Embedding
LacZ Staining of Nodules